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A. Why dilute?
Here are two situations that arise repeatedly in molecular biology labs:
1. You have a stock solution of some compound, let’s say an antibiotic, and you want to add the compound to growth medium, at a much smaller concentration than the stock solution.
2. You have a tube of very concentrated bacteria, perhaps a billion cells per milliliter. You want to put a few hundred of them on a petri plate, so that the colonies that arise will be easily distinguishable.
In both cases, the way out of the problem is to dilute the original solution. If you work in a lab, you need to know how to do this. Baca lebih lanjut
Ahmad Najib,1 Gemini Alam2 and Musdalifah Halidin1
1Faculty of Pharmacy, Moslem Universty of Indonesia
2Faculty of Pharmacy, Hasanuddin University
Plantago major L is a plant of the Plantaginaceae family which has been investigated on diethyl ether extract generate active spot as an antibacterial. The aim of the investigation was to identify the antibacterial compound from diethyl ether extract of daun sendok. as much 500 gram material was extracted maceration method was obtained 50 gram methanol extract, continued by partition with diethyl ether obtained 19.5 gram dried extract, the extract diethyl ether which isolated by suction column chromatography obtained 3 fractions (F1, F2 and F3). The third fraction (F3) was isolated by column chromatography obtained 2 fractions (3-1 and 3-2). The 3-1 active fraction (inhibit the Escherichia coli) isolated by preparative-TLC obtained 2 compounds. Compound 1 was active, single spot and visualization with Lieberman-Buchard and vanillin-sulfate acid. It have a steroid compound.
Keywords: Plantago major L, antibacterial, diethyl ether extract.
Source : Pharmacognosy Journal, 2012; 4(31):59-62
Departemen Farmasi FMIPA-UI
Each analysis method by some reason, must be validated. The parameters are selectivity, accuracy, precision, linearity, LOD, LOQ, ruggedness, and robustness. The parameters need to be calculated by assay methods. This paper try to give some information above these methods base on some literatures (USP 23rd, WHO, journal, etc).
Key words : Validation method, Parameter, Assay and calculation methods.
Sumber : Majalah Ilmu Kefarmasian, Vol. I, No.3, Desember 2004, 117 – 135
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