Category Archives: Bahan Kuliah

2013 in review

The WordPress.com stats helper monkeys prepared a 2013 annual report for this blog.

Here’s an excerpt:

The Louvre Museum has 8.5 million visitors per year. This blog was viewed about 110,000 times in 2013. If it were an exhibit at the Louvre Museum, it would take about 5 days for that many people to see it.

Click here to see the complete report.

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Hybridization

Hybridisation

Click the link for animation : http://www.mhhe.com/physsci/chemistry/essentialchemistry/flash/hybrv18.swf

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How to Dilute a Stock Solution

A. Why dilute?
Here are two situations that arise repeatedly in molecular biology labs:
1. You have a stock solution of some compound,  let’s say an antibiotic, and you want to add the compound to growth medium, at a much smaller concentration than the stock solution.
2. You have a tube of very concentrated bacteria, perhaps a billion cells per milliliter. You want to put a few hundred of them on a petri plate, so that the colonies that arise will be easily distinguishable.
In both cases, the way out of the problem is to dilute the original solution. If you work in a lab, you need to know how to do this. Baca lebih lanjut

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Isolation and identification of antibacterial compound from diethyl ether extract of Plantago major L

Ahmad Najib,1 Gemini Alam2 and Musdalifah Halidin1

1Faculty of Pharmacy, Moslem Universty of Indonesia
2Faculty of Pharmacy, Hasanuddin University

Abstract:

Plantago major L is a plant of the Plantaginaceae family which has been investigated on diethyl ether extract generate active spot as an antibacterial. The aim of the investigation was to identify the antibacterial compound from diethyl ether extract of daun sendok. as much 500 gram material was extracted maceration method was obtained 50 gram methanol extract, continued by partition with diethyl ether obtained 19.5 gram dried extract, the extract diethyl ether which isolated by suction column chromatography obtained 3 fractions (F1, F2 and F3). The third fraction (F3) was isolated by column chromatography obtained 2 fractions (3-1 and 3-2). The 3-1 active fraction (inhibit the Escherichia coli) isolated by preparative-TLC obtained 2 compounds. Compound 1 was active, single spot and visualization with Lieberman-Buchard and vanillin-sulfate acid. It have a steroid compound.

Keywords: Plantago major L, antibacterial, diethyl ether extract.

Source : Pharmacognosy Journal, 2012; 4(31):59-62

 

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Petunjuk Pelaksanaan Validasi Metode Dan Cara Perhitungannya

Harmita
Departemen Farmasi FMIPA-UI

ABSTRACT
Each analysis method by some reason, must be validated. The parameters are selectivity, accuracy, precision, linearity, LOD, LOQ, ruggedness, and robustness. The parameters need to be calculated by assay methods. This paper try to give some information above these methods base on some literatures (USP 23rd, WHO, journal, etc).
Key words : Validation method, Parameter, Assay and calculation methods.

Sumber : Majalah Ilmu Kefarmasian, Vol. I, No.3, Desember 2004, 117 – 135

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What is pharmacovigilance?

The WHO publication entitled The importance of pharmacovigilance: safety monitoring of medicinal products, describes pharmacovigilance as follows.
Pharmacovigilance is the science and activities relating to the detection, assessment, understanding and prevention of adverse effects of drugs or any other possible drug-related problems.
Recently, its concerns have been widened to include:
♦ herbals
♦ traditional and complementary medicines
♦ blood products
♦ biologicals
♦ medical devices
♦ vaccines.
Many other issues are also of relevance to the science:
♦ substandard medicines
♦ medication errors
♦ lack of efficacy reports
♦ use of medicines for indications that are not approved and for which there is inadequate scientific basis Baca lebih lanjut

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The dynamic relationship between the drug, the drug product, and the pharmacologic effect

Scheme demonstrating the dynamic relationship between the drug, the drug product, and the pharmacologic effect.

Click picture to enlarge

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Structure Elucidation of Disaccharides

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Quality Control Methods in Evaluating Herbal Medicinal Products

Quality control methods in evaluating herbal medicinal products.

Quality control methods in evaluating herbal medicinal products.

(Click picture to enlarge)

Source : Mukherjee, P.K., Houghton, P.J. (2009). ‘Evaluation of Herbal Medicinal Products Perspectives on quality, safety and efficacy’. London: Pharmaceutical Press.

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Introduction to Plant Anatomy

Plant Anatomy

Source : Great Valley High School

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Kromatografi

HPLC

HPLC

  • Defenisi
  • Kromatografi adalah teknik pemisahan campuran didasarkan atas perbedaan distribusi dari komponen-komponen campuran tersebut diantara dua fase, yaitu fase diam (padat atau cair) dan fase gerak (cair atau gas).

    Bila fase diam berupa zat padat yang aktif, maka dikenal istilah kromatografi penyerapan (adsorption chromatography). Bila fase diam berupa zat cair, maka teknik ini disebut kromatografi pembagian (partition chromatography).

  • Jenis-Jenis Kromatografi
  • Berdasarkan fase gerak yang digunakan, kromatografi dibedakan menjadi dua golongan besar yaitu gas chromatography dan liquid chromatography. Masing-masing golongan dapat dibagi lagi seperti yang telah disebutkan pada definisi di atas. [Selanjutnya...]

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